SKU: PCL-Methylene Blue 5mg/ml 20 mlCategories: Peptides, Research
What is Methylene Blue?
Methylene blue (MB), also known as methylthioninium chloride, is a synthetic heterocyclic aromatic compound with the molecular formula C₁₆H₁₈ClN₃S and a molecular weight of 319.85 g/mol (NIST Chemistry WebBook). It belongs to the phenothiazinium class of dyes and exhibits a characteristic blue color in its oxidized form (Nedu et al. 2020).
MB is redox-active, undergoing reversible conversion between its oxidized (blue) and reduced (colorless leucomethylene blue) states. This property underlies its use as a redox probe in chemical and biological research, including electron transport chain assays and mitochondrial function studies (Nedu et al. 2020). As an electron shuttle, MB can substitute for natural cofactors in redox reactions due to its favorable redox potential (Nedu et al. 2020).
Due to its cationic structure, MB binds electrostatically to negatively charged nucleic acids, making it widely used as a nucleic acid stain in many biochemical research procedures (Nedu et al. 2020). Its optical properties and low toxicity also make it suitable for confocal fluorescence microscopy and other imaging techniques (Nedu et al. 2020).
Photodynamically, MB serves as a dual-action photosensitizer. Upon excitation with visible light, it can undergo both Type I (radical-mediated) and Type II (singlet oxygen-mediated) photochemical reactions. These mechanisms form the basis of its use in photodynamic therapy research targeting tumor cells (Tardivo et al. 2005). The dye’s absorption in the therapeutic window (600–950 nm) makes it effective for light-based applications using red or polychromatic sources (Tardivo et al. 2005).
MB is also known for its good tissue penetration, attributed to its low molecular weight and amphiphilic character. This facilitates cellular uptake and accumulation in organelles like mitochondria, where its positive charge drives electrostatic localization in the mitochondrial matrix (Nedu et al. 2020). These properties support its use in perfusion mapping, tissue staining, and potential light-triggered therapeutic applications (Nedu et al. 2020; Tardivo et al. 2005).
Nedu, M.-E., et al. (2020). Comparative Study Regarding the Properties of Methylene Blue and Proflavine and Their Optimal Concentrations for In Vitro and In Vivo Applications. Diagnostics, 10(4), 223. https://doi.org/10.3390/diagnostics10040223
Tardivo, J. P., et al. (2005). Methylene blue in photodynamic therapy: From basic mechanisms to clinical applications. Photodiagnosis and Photodynamic Therapy, 2(3), 175–191. https://doi.org/10.1016/S1572-1000(05)00097-9
Buy Methylene Blue | High-Purity Compound for Research
PekCura Labs supplies high-purity methylene blue (methylthioninium chloride) intended for laboratory and institutional research. This synthetic heterocyclic compound has been extensively studied in fields such as pharmacology, toxicology, and biochemistry.
Product Overview
Product Name: Methylene Blue (Methylthioninium Chloride)
CAS Number: 61-73-4
Molecular Formula: C16H18ClN3S
Molecular Weight: 319.85 g/mol
Form: Lyophilized powder
Purity: ≥99%
Intended Use: For laboratory research use only
What Is Methylene Blue?
Methylene blue is a synthetic thiazine dye first synthesized in the 19th century. It is water-soluble, yielding a blue solution, and is commonly used as a redox indicator in chemical and biological systems. The compound has been the subject of numerous research publications for its roles in cellular energetics, oxidative stress response, and enzyme modulation.
Research Areas Involving Methylene Blue
Cellular Metabolism and Neuroenergetics: Investigated in rodent and in vitro models for its role in supporting mitochondrial electron transfer. [View Study]
Photodynamic Therapy (PDT): Explored in vitro as a light-activated agent for selective photochemical interactions with targeted tissues. [View Review]
Diagnostic Imaging Applications: Evaluated in lymph node mapping during cancer surgeries due to its staining and localization properties. [View Study]
Redox Studies and Hemoglobin Research: Commonly used in studies focusing on methemoglobinemia due to its redox cycling properties. [PDF Source]
Storage and Handling
Before Reconstitution: Store lyophilized vials at -4°F (-20°C) in a dry, cool environment.
After Reconstitution: Refrigerate at 35.6°F to 46.4°F (2°C to 8°C) and use within 14 days.
Light Protection: Store away from direct sunlight and humidity.
Safety Notice
This compound is intended strictly for laboratory research use only. It is not approved for human or veterinary use. Proper lab safety protocols must be followed when handling. Do not ingest or inject. Store securely and dispose of according to institutional regulations.
Our methylene blue is available for immediate online purchase through our secure checkout system. Standard and bulk quantities are available upon request. Shipping is available within the U.S., and all orders include a Certificate of Analysis (COA).
Methylene blue, also known by its chemical name methylthioninium chloride, is a synthetic compound with a rich scientific legacy. It has been used in various medical and research contexts for over a century, particularly for its electron-accepting properties and its ability to engage in redox reactions.
In research settings, methylene blue is prized for its role as a redox indicator and as a tool for probing mitochondrial function, oxidative stress pathways, and more. This product is specifically formulated for laboratory and investigational use and is not intended for therapeutic or diagnostic use in humans or animals.
Chemical Composition
Molecular Formula and Weight
Chemical Formula: C₁₆H₁₈ClN₃S
Molecular Weight: 319.85 g/mol
This molecular profile reflects methylene blue’s complex structure as a phenothiazine derivative. Its unique chemical arrangement enables it to interact with biological molecules through electron transfer and redox cycling.
The IUPAC name provides the standardized international nomenclature for chemical substances, while the CAS number is an identifier used globally to reference this specific compound in scientific literature and regulatory documentation.
Physical and Chemical Properties
Appearance and Solubility
Methylene blue appears as a dark green powder that produces a deep blue solution when dissolved in water. This coloration is due to its strong absorption properties in the visible light spectrum.
It is highly soluble in both water and ethanol, making it versatile for various types of research applications. The product dissolves quickly and evenly, allowing for consistent experimental conditions across different mediums.
Absorption Peak and Stability
The absorption peak of methylene blue occurs around 664 nm in aqueous solutions. This property is essential for spectrophotometric analysis and other optical detection methods used in scientific research.
The compound has a melting point of approximately 100–110°C, with decomposition occurring at higher temperatures. It should be stored in a cool, dark environment to preserve its chemical integrity over time.
Usage and Handling
Recommended Storage Conditions
For optimal stability, store methylene blue at room temperature in a dry, well-ventilated area away from direct light. Keep the container tightly sealed to prevent contamination or moisture absorption.
Avoid storing near acids or oxidizing agents, and ensure that handling procedures minimize exposure to environmental elements that could degrade the compound’s effectiveness.
Safety Precautions for Laboratory Use
This product is intended strictly for research use. Laboratory personnel should wear appropriate personal protective equipment (PPE), including gloves, lab coats, and eye protection when handling methylene blue.
In case of accidental contact or ingestion, follow institutional safety protocols and consult a safety data sheet (SDS) or a chemical safety officer for further guidance.
Quality Assurance
Purity Verification Methods
Our methylene blue undergoes rigorous testing to ensure it meets the highest purity standards for research-grade materials. Each batch is validated using advanced analytical techniques such as UV-Vis spectrophotometry and high-performance liquid chromatography (HPLC).
These methods confirm the identity, concentration, and absence of common contaminants, providing peace of mind for researchers who rely on accurate and reproducible data.
Accessing the Certificate of Analysis (COA)
A Certificate of Analysis (COA) is available for every batch of methylene blue we produce. The COA outlines critical metrics such as concentration, pH, sterility (where applicable), and expiration date.
You can request the COA directly on this product page or reach out to our customer support team. Having access to this documentation ensures traceability and compliance with institutional or grant-based research requirements.
Frequently Asked Questions
Is this methylene blue suitable for clinical applications?
No, this formulation is strictly intended for laboratory research use only. It is not approved for clinical, diagnostic, or therapeutic applications in humans or animals. Misuse may result in legal or safety violations.
How is this product different from dye-grade methylene blue?
Dye-grade methylene blue often contains impurities and stabilizers intended for textile or industrial use. In contrast, our research-grade formulation is purified and quality-tested to support sensitive biological and chemical studies.
Choosing research-grade ensures minimal interference in assays and a more controlled experimental environment, especially in redox studies, mitochondrial assays, or cellular imaging applications.
Legal & Regulatory Info
Research Use Only Disclaimer
This product is labeled and sold strictly for Research Use Only (RUO). It is not intended for use in diagnostic procedures, therapeutic interventions, or clinical applications. It must not be administered to humans or animals under any circumstances.
Customers are responsible for verifying that the use of this product complies with all applicable local, state, and federal regulations. Misuse may result in serious legal and safety consequences.
Compliance and Documentation
PekCura Labs adheres to stringent internal quality control protocols and ensures full documentation is available for regulatory or institutional review. This includes access to product specifications, batch history, and Certificates of Analysis (COAs).
While this product is not regulated under FDA drug pathways due to its non-clinical status, it meets internal manufacturing standards consistent with laboratory research requirements.
Source Quality and Purity
High-Purity Formulation
Our methylene blue is synthesized under controlled laboratory conditions and filtered to remove potential contaminants. Each production batch undergoes multi-stage purification to ensure consistent quality and optimal performance in precision research settings.
We maintain internal benchmarks for purity, concentration, and solubility to exceed typical research-grade expectations.
Supplier and Manufacturing Transparency
PekCura Labs partners exclusively with verified and audited suppliers for all base materials. The final product is manufactured in a U.S. based laboratory that follows strict quality assurance protocols.
Full documentation regarding sourcing, handling, and quality validation is available upon request to support compliance with academic, commercial, or governmental research programs.
Why Buy from Us?
Trusted Research Supplier
PekCura Labs is dedicated to supporting researchers with high-quality, well-documented compounds. Our methylene blue is formulated for consistency, tested for purity, and backed by real-time customer support.
We understand the importance of accuracy and reproducibility in scientific work. That’s why our products are accompanied by Certificates of Analysis (COAs), detailed specifications, and prompt fulfillment from our U.S. based facility.
Fast Shipping and Responsive Support
Orders are processed quickly—usually within 24 hours—and shipped with tracking to ensure your materials arrive promptly and securely. Our knowledgeable support team is ready to answer questions about your order or documentation needs.
Whether you're placing a one-time order or sourcing for an ongoing study, PekCura Labs delivers the reliability and transparency that research demands.
Chemical Composition of Methylene Blue
The methylene blue solution contains USP grade methylene for precision research and analysis.
Buy Methylene Blue Online – USP Grade
Why Choose USP Grade Methylene Blue?
USP Grade Methylene Blue ensures the highest level of purity and consistency. It is manufactured following strict standards, which makes it suitable for laboratory, research, and diagnostic applications. Choosing research grade means you're opting for a product that meets rigorous quality benchmarks and regulatory compliance. Each batch undergoes purity testing and is supported by documentation upon request.
Benefits of Ordering Direct from PekCura Labs
When you order directly from PekCura Labs, you’re choosing transparency, reliability, and fast fulfillment. We maintain direct control over sourcing, storage, and shipping to ensure the integrity of each product. Our secure checkout, dedicated customer support, and responsive fulfillment team make the process smooth from start to finish.
Monthly Coupon Codes & Order Perks
Each month, we feature updated coupon codes visible at the top of every page, giving our customers rotating opportunities to save. These codes can be applied at checkout for added value. In addition, every order is backed by our quality assurance commitment, and eligible purchases may qualify for free shipping or promotional bonuses.
Trusted Methylene Blue Suppliers & Options
How to Select a Reliable Source
Not all methylene blue products are created equal. When evaluating a supplier, it's essential to verify product grade, batch testing, and manufacturing transparency. Look for sources that offer research-grade material, clearly label ingredients, and provide access to documentation. Suppliers should also follow proper handling and storage procedures to maintain product integrity during transit.
Methylene Blue Availability and Lead Times
We keep our inventory up to date to ensure fast order processing and delivery. Typical lead times for most orders are 1–2 business days, depending on order volume and shipping destination. In the rare event of a delay, our team will communicate promptly and provide updates every step of the way. If you're ordering for time-sensitive applications, feel free to reach out for expedited support.
For Bulk Orders: Contact Us Directly
If you're interested in bulk purchasing or wholesale inquiries, we encourage you to connect with us directly. Please email us at contact@pekcuralabs.com with your specific requirements, volume estimates, and intended use. Our team will provide a tailored quote and fulfillment plan to meet your needs.
Chemical Composition of Methylene Blue
Molecular Structure and IUPAC Name
Methylene blue, known chemically as Methylthioninium chloride, belongs to the phenothiazine family of compounds. Its IUPAC name is 3,7-bis(dimethylamino)-phenothiazin-5-ium chloride. The molecule features a phenothiazine core with dimethylamino groups at positions 3 and 7, contributing to its deep blue coloration and redox properties.
CAS Number, Formula, and Molecular Weight
The CAS Registry Number for methylene blue is 61-73-4. Its molecular formula is C₁₆H₁₈ClN₃S, and it has a molecular weight of 319.85 g/mol. This compound is typically provided as a chloride salt and is widely used in chemistry, biology, and clinical research settings.
Physical Properties and Solubility
Methylene blue appears as a dark green crystalline powder that produces a vibrant blue solution when dissolved. It is highly soluble in water and ethanol. The compound has a melting point of approximately 100–110°C (with decomposition) and an absorption peak at around 664 nm in aqueous solutions, attributes that contribute to its role as a staining agent and its visibility in solution-based diagnostics.
Scientific Research on Methylene Blue
Studies Exploring Its Role in Redox Reactions
Methylene blue is widely studied for its redox activity, particularly its ability to act as an electron donor or acceptor. In biomedical contexts, it is known to facilitate electron transfer in mitochondrial pathways and oxidative stress models. Numerous peer-reviewed studies have evaluated its efficacy in supporting cellular function through redox modulation.
Scientific interest in methylene blue spans neurology, infectious disease, and pharmacology. Researchers have explored its potential roles in addressing methemoglobinemia, neurodegenerative disorders, and as a surgical staining agent. All medical applications should be evaluated by professionals and guided by evidence-based literature.
While methylene blue has demonstrated value in many research domains, users should always consult applicable regulations and review existing safety data. Usage in humans or animals must follow proper protocols, and any off-label or investigational use should be discussed with a qualified professional.
What’s the Difference Between USP and Industrial Grades?
USP-grade methylene blue is produced under stringent quality standards to ensure high purity and consistency, making it ideal for laboratory and diagnostic applications. Industrial-grade alternatives may contain impurities or inconsistent formulation, and are not suitable for precise research environments.
Is Methylene Blue Regulated or Prescription-Based?
Methylene blue is not considered a controlled substance in most regions when used for research, industrial, or non-clinical applications. However, its use in medical treatment may require oversight, depending on local regulations. Always consult applicable guidelines before use.
Where Can I Find Documentation or Lab Reports?
Each batch of methylene blue from PekCura Labs is tested for purity and quality. Upon request, we can provide Certificates of Analysis (COA) and other supporting documents. If you need documentation for compliance, research, or institutional review, please contact our team.
Need Help or Have Questions?
We're Here to Support You
If you have any questions about our methylene blue products, usage guidelines, documentation, or order details, our support team is ready to help. We aim to respond to all inquiries within 1 business day. Please don’t hesitate to reach out via email for fast, personalized assistance.
Committed to Transparency and Quality
At PekCura Labs, we believe that trust begins with transparency. That’s why we make our sourcing, testing procedures, and documentation accessible to all qualified buyers. Whether you're ordering for the first time or managing a bulk research project, you can count on consistent quality and clear communication.
Looking for More Information?
Visit our FAQ page or explore the research links above to learn more about the properties and scientific background of methylene blue. For direct support, don’t hesitate to email contact@pekcuralabs.com.
What Makes a High-Quality Methylene Blue Solution?
Why Researchers Trust Research Grade Methylene Blue
Product Usage
Product Usage: This PRODUCT IS INTENDED AS A RESEARCH CHEMICAL ONLY. This designation allows the use of research chemicals strictly for in vitro testing and laboratory experimentation only. All product information available on this website is for educational purposes only. Bodily introduction of any kind into humans or animals is strictly forbidden by law. This product should only be handled by licensed, qualified professionals. This product is not a drug, food, or cosmetic and may not be misbranded, misused or mislabeled as a drug, food or cosmetic.
