Why Choose Us?

3

Research-Grade
Purity

All peptides have a guaranteed purity of at least 99%.

2

Manufactured in
the USA

Our top-quality products are manufactured right here in the United States.

1

Fast
Shipping

Your products arrive at your door quickly. Plus, shipping is free for orders over $100.

4

Guaranteed
Freshness

Products are stored at -20° and shipped in cool shield bubble mailers.

5

Independently
Tested

Each batch is independently tested to ensure product purity and dosage.

Why Choose Us?

3

Research-Grade
Purity

All peptides have a guaranteed purity of at least 99%.

2

Manufactured in
the USA

Our top-quality products are manufactured right here in the United States.

1

Fast
Shipping

Your products arrive at your door quickly. Plus, shipping is free for orders over $100.

4

Guaranteed
Freshness

Products are stored at -20° and shipped in cool shield bubble mailers.

5

Independently
Tested

Each batch is independently tested to ensure product purity and dosage.

Methylene Blue Vial Transparent Vial Only

What is Methylene Blue?

Methylene blue (MB), also known as methylthioninium chloride, is a synthetic heterocyclic aromatic compound with the molecular formula C₁₆H₁₈ClN₃S and a molecular weight of 319.85 g/mol (NIST Chemistry WebBook). It belongs to the phenothiazinium class of dyes and exhibits a characteristic blue color in its oxidized form (Nedu et al. 2020).

MB is redox-active, undergoing reversible conversion between its oxidized (blue) and reduced (colorless leucomethylene blue) states. This property underlies its use as a redox probe in chemical and biological research, including electron transport chain assays and mitochondrial function studies (Nedu et al. 2020). As an electron shuttle, MB can substitute for natural cofactors in redox reactions due to its favorable redox potential (Nedu et al. 2020).

Due to its cationic structure, MB binds electrostatically to negatively charged nucleic acids, making it widely used as a nucleic acid stain in many biochemical research procedures (Nedu et al. 2020). Its optical properties and low toxicity also make it suitable for confocal fluorescence microscopy and other imaging techniques (Nedu et al. 2020).

Photodynamically, MB serves as a dual-action photosensitizer. Upon excitation with visible light, it can undergo both Type I (radical-mediated) and Type II (singlet oxygen-mediated) photochemical reactions. These mechanisms form the basis of its use in photodynamic therapy research targeting tumor cells (Tardivo et al. 2005). The dye’s absorption in the therapeutic window (600–950 nm) makes it effective for light-based applications using red or polychromatic sources (Tardivo et al. 2005).

MB is also known for its good tissue penetration, attributed to its low molecular weight and amphiphilic character. This facilitates cellular uptake and accumulation in organelles like mitochondria, where its positive charge drives electrostatic localization in the mitochondrial matrix (Nedu et al. 2020). These properties support its use in perfusion mapping, tissue staining, and potential light-triggered therapeutic applications (Nedu et al. 2020; Tardivo et al. 2005).

References

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